Development and Application of Calibration Device for Fluorescence Quantitative PCR Analyzer

Abstract

This paper proposes a PWM-based light intensity modulation method, and develops a calibration device for fluorescence quantitative polymerase chain reaction (PCR) analyzer. To verify the performance of this calibration device, a temperature calibration system composed of isothermal block, standard platinum resistance thermometer, and thermostatic bath was employed in experimental measurements. Results showed that the absolute value of the indication errors did not exceed 0.05°C, and the uniformities did not exceed 0.06°C. Additionally, the linearity of LED emitting intensity versus relative intensity verified using a luminance meter showed a good fitting coefficient R2≥0.9999. Ultimately, this calibration device was used to calibrate the temperature and optical physical parameters of the fluorescence quantitative PCR analyzer, comprehensively evaluating its technical specifications.